Cell Line Development Research Associate
Cell Line Development Research Associate is involved in the creation of stable cell lines for the recombinant adeno-associated viral vector (rAAV) production processes . He/she executes experiments, produces relevant documentation, and works under supervision. He/she will be responsible for:
• Culturing mammalian cells for viral production in shake flasks, spinner flasks, spin tubes, deep well and automation platforms.
• Performing experiments in the development, optimization and scale-up of AAV producer cell lines.
• Preparing process development protocols, data analysis summaries, technical reports, and other relevant process documentation.
• Optimization of transfection and transduction protocols for newly engineered cell lines.
• Maintaining an accurate laboratory notebook.
• Ensuring work conforms to applicable quality and safety standards.
• Participating on project teams (as needed).
B.S. with 3-6 years of experience, MS.c with 2-5 years of relevant scientific or technical experience in the life sciences industry or equivalent
• Experience in cell culture including materials, instruments and technologies is essential.
• Experience with viral vector production is preferred . Key Skills, Abilities, and Competencies:
Hands-on experience in mammalian cell culture production processes is a critical requirement. This includes small scale (shaker flasks, spinner flasks, spin tubes, deep well) and/or benchtop bioreactor scale.
Experience with creation, analysis and characterization of new engineered cell lines and stability studies (subcloning, clonal expansion, transient transfection and expression screening) is a strong plus.
Experience in molecular biology techniques (plasmid design, molecular cloning, SDS-PAGE/western blotting, qPCR, etc) is a strong plus.
Automation and ELN experience is a strong plus.
Effective communication skills (verbal, written, interpersonal).
Industry experience is a plus but not required
Basic Knowledge on AAV biology is a plus.
Must be able to interact with cross-functional teams.
Analytical and problem-solving skills.
Ability to manage time effectively.
Experience in laboratory automation hardware and software is a plus.
Teamwork is a must. Must be able to lift 25 pounds.
• Due to the inherent nature of cell culture, some weekend work and/or work on holidays may be needed. On occasion, long work hours may be required depending upon the experiment schedule.
• Handling large volumes of cell culture or related materials, such as tens of liters, may be required. Also, several viral materials, including AAV may be involved in the work. Pay Rate Range: $30-40/hr depending on experience
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