Lab Technician
- Duration: 6 months
- Employment: Full time
- Location: Pittsburgh area, as this is a fully on-site role
Working to support the CAR-T screening cascade, this role will support multiple teams and projects in the discovery pipeline. Your primary responsibility will be maintaining primary cell cultures for screening assays, but will also include but are not limited to the following:
- Primary human cell culture
- Primary T-cell activation, transduction, and maintenance
- Cytotoxicity and cytokine release assays
- Method development and optimization for cell-based assays
- qPCR
- Flow cytometry
- Lentivirus production and titration
- Various other procedures not mentioned herein
- Bachelor’s degree in a scientific field
- 2+ years of experience as laboratory technician or in a comparable position
- Advanced cell culture skills: primary cell culture experience is a must, CART experience is a plus
- Ability to coordinate multiple tasks simultaneously
- Experience in cell-based assays is a plus
- Exceptional attention to detail
- Ability to work independently with minimal supervision
- Organized and accurate in your work but also flexible towards changing plans
- Desire to learn new concepts and experimental procedures
- Desire to learn how to solve complex problems with limited information
- Ability to implement and independently interpret results of cellular assays for analysis of biological function and pharmacological evaluation of CAR-Ts or antibodies
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